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Simultaneous Flow Cytometric Analysis of Phagocytosis and Oxidative Burst Activity in Equine Leukocytes

✍ Scribed by M.J.B.F. Flaminio; B.R. Rush; E.G. Davis; K. Hennessy; W. Shuman; M.J. Wilkerson

Book ID
110326591
Publisher
Springer Netherlands
Year
2002
Tongue
English
Weight
224 KB
Volume
26
Category
Article
ISSN
0165-7380

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A one-step flow cytometric (FCM) assay has been developed to quantify both opsonin-and antigendependent phagocytosis and intraphagocyte oxidative burst responses. Meningococcal outer membrane structures (OMV) were adsorbed to fluorescent polystyrene beads, opsonized with serum, and exposed to leukoc