We investigated and compared the DNA content between primary and metastatic lesions from 30 colorectal cancer cases. The findings were only diploid in 2 cases (6.7%) and only aneuploid in 18 cases (60.0%). On the whole, the DNA ploidy agreed in 20 cases (66.7%) between both lesions. In the 18 cases
Flow cytometric analysis of DNA stemline heterogeneity in primary and metastatic breast cancer
β Scribed by Henk Beerman; Vincent T. H. B. M. Smit; Philip M. Kluin; Bert A. Bonsing; Jo Hermans; Cees J. Cornelisse
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 685 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0196-4763
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## Abstract Flowβcytometric DNA analyses of single cell nuclei were performed on nuclear suspensions prepared from biopsies of primary breast carcinomas in 638 patients. Propidiumβiodideβstained cell nuclei were analysed in an Ortho 50βH Cytofluorograph. The patients were staged by the TMN classifi
DNA histograms were measured by flow cytometry for 656 human solid tumors (365 primary and 291 metastatic). The proportion of aneuploid cells in cell suspensions obtained by mechanical disaggregation was significantly higher than those obtained after enzymatic disaggregation (collagenase + DNAse) of
The percentage of cells in S-phase and DNA-ploidy have been measured in 300 primary mammary carcinomas by means of DNA-flow cytometry (FCM). The data were compared with the age and menopausal status of the patients as well as with the size, regional lymph-node involvement, histologic type, grade and