Fixation and Permeabilization of Cells and Tissues
โ Scribed by Fischer, A. H.; Jacobson, K. A.; Rose, J.; Zeller, R.
- Book ID
- 115481286
- Publisher
- Cold Spring Harbor Laboratory Press
- Year
- 2008
- Tongue
- English
- Weight
- 76 KB
- Volume
- 2008
- Category
- Article
- ISSN
- 1940-3402
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Cell death is normally accompanied by loss of integrity of the cell membrane. Concomitant loss of osmotic pressure and permeability for DNA binding dyes like propidium iodide make it possible to distinguish viable from nonviable cells. However, after permeabilization for intracellular staining or af
the authors want to correct the Methods section regarding the fixation/permeabilization protocols used for this study. The authors mentioned the use of 4% paraformaldehyde (PFA) on pages 2 and 3 to resuspend the cells lasting the final step before flow acquisition, when in reality 1% PFA was used. F