Fiftyfold amplification of the Lowry protein assay
โ Scribed by Michael G. Sargent
- Book ID
- 102628703
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 488 KB
- Volume
- 163
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The Lowry protein assay was directly adapted for use on the Cobas-Bio centrifugal analyzer resulting in a 50% reduction of the coefficients of variation of the manual method. It was also demonstrated that the reaction need not be pursued to its endpoint and that absorbance readings taken immediately
for protein concentration measurement has been automated to permit assay of samples with concentrations from 1 to 400 @g/ml. Calibration with solutions of bovine serum albumin resulted in a nonlinear (quadratic) curve. The quantity of color developed in the assay was found to be strongly dependent o