✦ LIBER ✦

Optimization of Folin-Ciocalteu reagent concentration in an automated Lowry protein assay

✍ Scribed by G.M. Oosta; N.S. Mathewson; G.N. Catravas

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 197 KB
Volume: 89
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90723-6

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✦ Synopsis

for protein concentration measurement has been automated to permit assay of samples with concentrations from 1 to 400 @g/ml. Calibration with solutions of bovine serum albumin resulted in a nonlinear (quadratic) curve. The quantity of color developed in the assay was found to be strongly dependent on the concentration of the Folin-Ciocalteu phenol reagent. Color yield peaked sharply at a reagent concentration 40% lower than that used in the Lowry procedure. Optimization of the reagent concentration is necessary to obtain maximum sensitivity from the Lowry assay.