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Development of an automated Lowry protein assay for the Cobas-Bio centrifugal analyzer

✍ Scribed by P.M. Clifton; L. Chang; A.M. Mackinnon

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 294 KB
Volume: 172
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90426-5

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✦ Synopsis

The Lowry protein assay was directly adapted for use on the Cobas-Bio centrifugal analyzer resulting in a 50% reduction of the coefficients of variation of the manual method. It was also demonstrated that the reaction need not be pursued to its endpoint and that absorbance readings taken immediately after the addition of the Folin reagent were directly related to the protein concentration. Thus the total assay timecould be reduced from 30 to 3 min with no loss of accuracy or reproducibility.

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