Fidelity genotyping of point mutation by enhanced melting point difference using DNA ligase
โ Scribed by Xiangxian Meng; Huimin Li; Kemin Wang; Weihong Tan; Jun Li; Qiuping Guo; Xiaohai Yang; Qiuhua Mo; Xiangmin Xu
- Book ID
- 116902366
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 780 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0039-9140
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## Abstract The present study reports a proofโofโprinciple for a sensitive genotyping assay approach that can detect single nucleotide polymorphisms (SNPs) based on fluorescence anisotropy measurements through a coreโshell fluorescent nanoparticles assembly and ligase reaction. By incorporating the
A novel strategy is described for highly sensitive DNA detection and point mutation identification based on the combination of reverse molecular beacon with DNA ligase. A 5'-phosphoryl and 3'-ferrocene terminated DNA sequence is used as detection probe, which may be ligated to capture DNA immobilize