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Fertile plant regeneration from cell suspension and protoplast cultures of barley (t Hordeum vulgare cv. Schooner)

✍ Scribed by Rohan R. Singh; Jodie A. Kemp; James F. Kollmorgen; Javed A. Qureshi; Geoffrey B. Fincher


Book ID
110229805
Publisher
Springer
Year
1997
Tongue
English
Weight
210 KB
Volume
49
Category
Article
ISSN
0167-6857

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Protoplasts were isolated from immature inflorescence-derived embryogenic suspension cultures of two cultivars of Sorghum vulgare. The protoplasts were cultured in a modified K8P liquid medium. They started to divide after 4-5 days of culture, and achieved 16.8% division frequency by 10 days. Protoc