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Fertile plant regeneration from barley (Hordeum vulgare L.) protoplasts isolated from primary calluses

✍ Scribed by Makoto Kihara; Hideyuki Funatsuki


Book ID
119137442
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
606 KB
Volume
106
Category
Article
ISSN
0168-9452

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Regeneration of fertile plants from embr
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Protoplasts were isolated from immature inflorescence-derived embryogenic suspension cultures of two cultivars of Sorghum vulgare. The protoplasts were cultured in a modified K8P liquid medium. They started to divide after 4-5 days of culture, and achieved 16.8% division frequency by 10 days. Protoc