Feedback regulation of RNA polymerase subunit synthesis after the conditional overproduction of RNA polymerase in Escherichia coli

✍ Scribed by Bedwell, David M. ;Nomura, Masavasu

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 794 KB
• Volume: 204
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00330181

✦ Synopsis

The beta and beta' subunits of RNA polymerase are thought to be controlled by a translational feedback mechanism regulated by the concentration of RNA polymerase holoenzyme. To study this regulation in vivo, an inducible RNA polymerase overproduction system was developed. This system utilizes plasmids from two incompatibility groups that carry RNA polymerase subunit genes under lac promoter/operator control. When the structural genes encoding the components of core RNA polymerase (alpha, beta and beta') or holoenzyme (alpha, beta, beta' and sigma 70) are present on the plasmids, induction of the lac promoter results in a two fold increase in the concentration of functional RNA polymerase. The induction of RNA polymerase overproduction is characterized by an initial large burst of beta beta' synthesis followed by a gradual decrease as the concentration of RNA polymerase increases. Overproduction of RNA polymerase in a strain carrying an electrophoretic mobility mutation in the rpoB gene results in the specific repression of beta beta' synthesis off the chromosome. These results indicate that RNA polymerase feedback regulation controls beta beta' synthesis in vivo.