Synthesis of RNA polymerase subunits and of transcription termination factor p was studied after thermoinduction of prophage lambdac1857 located at several unusual sites on the chromosome of Escherichia coli. When a lysogen carrying the prophage at the bfe gene was induced at 42 degrees C, the rate
Induction of RNA polymerase synthesis in Escherichia coli
โ Scribed by Glass, Robert E. ;Goman, Michael ;Errington, Laurence ;Scaife, John
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 512 KB
- Volume
- 143
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
Studies on the rate of synthesis of the beta and beta' subunits of RNA polymerase in haploid strains of Escherichia coli K12 containing poorly-suppressed rif degrees am mutations provide conclusive evidence that synthesis of at least these two subunits is regulated.
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Temperature-sensitive mutants of Escherichia coli that are unable to grow at high temperature can be obtained among those selected for resistance to streptovaricin or rifampicin at low temperature (Yura et al., 1973). One of these mutants (KY5323) that was supposed to carry a single mutation affecti