Fast HPLC method for the determination of glimepiride, glibenclamide, and related substances using monolithic column and flow program

✍ Scribed by Sami El Deeb; Udo Schepers; Hermann Wätzig

• Book ID: 102926473
• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 723 KB
• Volume: 29
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600056

✦ Synopsis

Fast HPLC method for the determination of glimepiride, glibenclamide, and related substances using monolithic column and flow program This work presents a fast method for the simultaneous separation and determination of glimepiride, glibenclamide, and two related substances by RP LC. The separation was performed on a Chromolith Performance (RP-18e, 100 mm64.6 mm) column. As mobile phase, a mixture of phosphate buffer pH 3, 7.4 mM, and ACN (55 : 45 v/v) was used. Column oven temperature was set to 308C. The total chromatographic run time was 80 s. This was achieved using a flow program from 5 to 9.9 mL/min. Precisions of the interday and the intraday assay for both retention times and peak areas for the four analyzed compounds were less than 1.2%. The method showed good linearity and recovery. The short analysis time makes the method very valuable for quality control and stability testing of drugs and their pharmaceutical preparations.