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Fast atom bombardment-collision activated dissociation-linked field scanning mass spectrometry of the neuropeptide substance P

✍ Scribed by Dominic M. Desiderio; Itsuo Katakuse

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
326 KB
Volume
129
Category
Article
ISSN
0003-2697

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✦ Synopsis

Amino acid sequence-determining information is obtained from nanomole amounts of the underivatized, biologically important peptide substance P by combining fast atom bombardment, collision activated dissociation, and linked field scanning mass spectrometry. Protonated molecular ions of substance P are produced by fast atom bombardment mass spectrometry, accelerated to high translational energy (8 kV), and transit a collision chamber. Collision activated dissociations occur in the first field-free region. Amino acid sequence-determining ions are collected by scanning the magnetic and electric fields, keeping their ratio constant. In this manner, the precursor-product relationship among ions produced during fragmentation of the protonated molecular ion is firmly established.

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