Enhanced structural information on oligosaccharides by scan of linked magnetic and electrostatic fields (B/E) and neutral gas collision fast-atom-bombardment mass spectrometry (FABMS)
✍ Scribed by Vladimír Kováčk; Hian Kee Lee; Pavol Kováč
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 526 KB
- Volume
- 239
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Homo-and hetero-o&saccharides l-27, composed of hexopyranose, deoxyhexopyranose, deoxyfluorohexopyranose, and Zacetamido-2deoxyhexopyranose units, have been examined by fast-atombombardment mass spectrometry. Scans by finked magnetic and electrostatic (B/E) fields of quasimolecular [M + HI+ ions, or scans combined with helium collision, gave rise to structurally significant ions. The information thus obtained aids significantly in the sequence analysis of oligosaccharides without derivatization.
INTRODUCPION
Mass spectrometry plays an important role in structural analysis of complex carbohydrates. The available methods, which involve the ionization in the gas phase, e.g., electron-impact (ED and chemical-ionization (CI) mass spectrometry, provide useful information for the confirmation of structures of substances that are sufficiently volatile or mixtures of substances amenable to gas-liquid or liquid chromatography. The low volatility of many substances makes their analysis by mass spectrometry impractical and requires prior chemical manipulation to produce a sufficiently volatile material. Such procedures may be difficult to apply with substances that are unstable or undergo side reactions or are available in only minute amounts.