False positive results occurring in a radioimmunoassay for hepatitis A IgM antibody of the IgM class
โ Scribed by Marion R. Bucens; Glenys R. Pietroboni; Gerald B. Harnett
- Book ID
- 119114134
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 549 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0166-0934
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๐ SIMILAR VOLUMES
## Abstract A solidโphase radioimmunoassay (RIA) for IgM antiโHBc is described. The assay (antiโmฬ RIA) is based on the adsorption of IgM to a tube coated with sheep antibody to the human IgM mฬโchain. The adsorbed immunoglobulin is assayed for antiโHBc activity. Positive reactions in the test are
A new test principle for the detection of specific IgM-class antibodies was developed and applied in an Enzyme-Linked Immuno Sorbent Assay (ELISA) for the detection of hepatitis A IgM antibodies. A solid phase coated with anti-IgM was incubated successively with serum sample, specific antigen, and e
## Abstract Three methods were compared for determining antiโHAV of the IgM class. In the first method flatโbottomed microtiter plates coated consecutively with antiโHAV of the IgG class and HAAg were incubated with patient serum and, after washing, peroxidase conjugated antiโmฬ was added. After su