𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis a

✍ Scribed by W. Duermeyer; F. Wielaard; J. van der Veen

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
407 KB
Volume
4
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

A new test principle for the detection of specific IgM-class antibodies was developed and applied in an Enzyme-Linked Immuno Sorbent Assay (ELISA) for the detection of hepatitis A IgM antibodies. A solid phase coated with anti-IgM was incubated successively with serum sample, specific antigen, and enzyme-labeled F (ab')2 fragments from IgG antibodies against the antigen and enzyme substrate. F(ab')2 fragments were used to avoid interference with rheumatoid factor. Specificity and sensitivity are very high. This test principle appears generally applicable in the diagnosis of infectious and parasitic diseases by testing only one serum sample.

πŸ“œ SIMILAR VOLUMES

A new microimmunofluorescence test for t
A new microimmunofluorescence test for the detection of Chlamydia pneumoniae specific antibodies
✍ F. FernΓ‘ndez; J. GutiΓ©rrez; J. Mendoza; J. Linares; M. J. Soto πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 114 KB

## Abstract To evaluate a microimmunofluorescence (MIF) test (__Chlamydia pneumoniae__ IgG, Vircell, Spain) that detects IgG against __Chlamydophila pneumoniae__ (Cp), MRL Diagnostics MIF was used as reference test. Cross‐reactivity against __Chlamydia trachomatis__ (Ct) and __Chlamydophila psittac

A modified passive-haemagglutination tec
A modified passive-haemagglutination technique for the detection of cytomegalovirus and herpes simplex virus antibodies: Application in virus-specific IgM diagnosis
✍ W. Al-Nakib πŸ“‚ Article πŸ“… 1980 πŸ› John Wiley and Sons 🌐 English βš– 437 KB πŸ‘ 2 views

Cytomegalovirus (CMV) and herpes simplex virus (HSV) antibodies were detected by a modified passive haemagglutination (PHA) technique. The main features of this modification are the use of a simpler method for the removal of nonspecific sheep agglutinins in the sera, the deployment of commercially a

Blocking-ELISA for detection of specific
Blocking-ELISA for detection of specific antibodies to the glycoproteins of the human immunodeficiency virus type 1 (HIV-1)
✍ J. C. Tardy; J. Basson; A. Douglas; M. Aymard πŸ“‚ Article πŸ“… 1990 πŸ› John Wiley and Sons 🌐 English βš– 403 KB πŸ‘ 1 views

## Abstract A blocking ELISA was developed to confirm the specificity of screening tests for anti‐HIV‐1 antibodies. A murine monoclonal antibody (McAb) raised against recombinant gp 160 was used in combination with a commercial technique (ELAVIA‐1). After determining the optimal experimental condit

The detection of mast cell subpopulation
The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase
✍ Buckley, Mark G.; McEuen, Alan R.; Walls, Andrew F. πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 353 KB πŸ‘ 2 views

Chymase is an important marker for human mast cells as well as a mediator of inflammation and matrix remodelling, but research into chymase-containing mast cell subpopulations has been hampered by the lack of reagents suitable for use with formalin-fixed tissue. A monoclonal antibody to chymase (des

Evaluation of a new commercial enzyme im
Evaluation of a new commercial enzyme immunoassay for the detection of IgM antibodies to West Nile virus using a ratio method to eliminate nonspecific reactivity
✍ Ryan J. Welch; Brian L. Anderson; Christine M. Litwin πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 101 KB

## Abstract As West Nile virus (WNV) has become endemic in the United States, following the first reported cases in New York during the summer of 1999, the demand for specific serology has increased. Several IgM capture ELISA assays for the detection of WNV‐specific IgM have been approved by the Fo