𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A new microimmunofluorescence test for the detection of Chlamydia pneumoniae specific antibodies

✍ Scribed by F. Fernández; J. Gutiérrez; J. Mendoza; J. Linares; M. J. Soto

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
114 KB
Volume
44
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

To evaluate a microimmunofluorescence (MIF) test (Chlamydia pneumoniae IgG, Vircell, Spain) that detects IgG against Chlamydophila pneumoniae (Cp), MRL Diagnostics MIF was used as reference test. Cross‐reactivity against Chlamydia trachomatis (Ct) and Chlamydophila psittaci (Cps) was investigated. Eighty sera were analysed from 22 subjects with vascular disease, 38 with multiple sclerosis and 20 healthy individuals. Vircell and MRL MIF tests assessed 58.75% and 60% of the samples as positive, respectively, and their results coincided (positive/negative) in 98.75% of samples. One major (>1 IgG titre) and 32 minor (1 titre) discrepancies were observed. Correlation between tests was significant. Vircell MIF test demonstrated 97.9% sensitivity and 100% specificity. Differences in simultaneous reactivity to Ct and Cps between the tests were not significant. Vircell MIF test showed a good performance to detect the IgG against Cp. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

📜 SIMILAR VOLUMES

A new gold standard for the detection of
A new gold standard for the detection of Chlamydia trachomatis?
✍ Gilles R.G. Monif 📂 Article 📅 1998 🏛 Hindawi Publishing Corporation 🌐 English ⚖ 40 KB 👁 2 views

C ell-culture techniques have long been consid- ered the ''gold standard'' for the detection of Chlamydia trachomatis. The major advantage of cellculture isolation is the specificity, which approaches 100%; however, even in experienced laboratories, its sensitivity is only 70-80%. 1-3 Its primary us

The EDB Test—A clinical test for the det
The EDB Test—A clinical test for the detection of antibodies to botulinum toxin type A
✍ Kirn R. Kessler; Dr. Reiner Benecke 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 427 KB

## Abstract Approximately 5 to 10% of patients with cervical, segmental or multifocal dystonia receiving repetitive local injections with botulinum toxin A (BTX A) are estimated to develop secondary loss of treatment benefit (nonresponding) because of the formation of circulating serum antibodies a

A new principle for the detection of spe
A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis a
✍ W. Duermeyer; F. Wielaard; J. van der Veen 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 407 KB

A new test principle for the detection of specific IgM-class antibodies was developed and applied in an Enzyme-Linked Immuno Sorbent Assay (ELISA) for the detection of hepatitis A IgM antibodies. A solid phase coated with anti-IgM was incubated successively with serum sample, specific antigen, and e

Simple haemadherence test for the detect
Simple haemadherence test for the detection of class-specific immunoglobulins to hepatitis A virus
✍ Mr. Keith R. Perry; Mr. John V. Parry 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 478 KB 👁 1 views

The ability of hepatitis A virus (HAV) to agglutinate human erythrocytes was used to develop IgM and IgG antibody capture haemadherence tests (MACHAT and GACHAT). Haemadherence was dependent on the pH of the red cell suspension and was best in the pH range 5.4 to 5.8. The tests were applied to serum

The detection of mast cell subpopulation
The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase
✍ Buckley, Mark G.; McEuen, Alan R.; Walls, Andrew F. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 353 KB 👁 2 views

Chymase is an important marker for human mast cells as well as a mediator of inflammation and matrix remodelling, but research into chymase-containing mast cell subpopulations has been hampered by the lack of reagents suitable for use with formalin-fixed tissue. A monoclonal antibody to chymase (des

The biochemical specific-locus test and
The biochemical specific-locus test and a new multiple-endpoint mutation detection system: Considerations for genetic risk assessment
✍ Dr. Susan E. Lewis 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 375 KB

The importance of studying the generation of mutotions to human genetic risk is also dismutations in mouse germ cells is emphasized, cussed. Finally, the value of using multiple tests and the utility of the biochemical specific-locus for the study of in vivo germinal mutations is test in detecting g