Factors affecting isolation of protoplasts from leaves of grape (Vitis vinifera)
β Scribed by Daniel C. Wright
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 299 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0167-6857
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π SIMILAR VOLUMES
Mature leaves of Secale cereale cut into narrow strips and incubated for 18 h in a mixture of cellulase (Meicelase) and pectinase (Pectinol R10) produced quantities of protoplasts. Under the same conditions leaves of Triticum aestivum, Hordeum vulgare and Avena sativa also produce protoplasts but in
## Abstract **BACKGROUND:** A fast and easy method to isolate RNA from grape (__Vitis vinifera__) berries is proposed. **RESULTS:** The procedure uses high concentrations of cetyltrimethylammonium bromide (CTAB) and includes boric acid with the purpose of increasing the RNA extraction. Eppendorf t