Isolation of protoplasts from sugar beet leaves
✍ Scribed by Li-Su-Nam; Běla Landová; Z. Landa
- Publisher
- Springer Netherlands
- Year
- 1976
- Tongue
- English
- Weight
- 466 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0006-3134
No coin nor oath required. For personal study only.
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Mature leaves of Secale cereale cut into narrow strips and incubated for 18 h in a mixture of cellulase (Meicelase) and pectinase (Pectinol R10) produced quantities of protoplasts. Under the same conditions leaves of Triticum aestivum, Hordeum vulgare and Avena sativa also produce protoplasts but in
Polyribosomes (polysomes) , active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/ monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated prot
The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast devel
Curves relating the viscous conductivity, Sv sec/cm, to the diffusive conductivity, So cm-1, have been plotted for leaves of sugar beet and potato with defined stomatal concentrations and dimensions. The relationships and the theory of porometers have been used to obtain estimates of diffusive condu