Factors affecting free zone electrophoresis and isoelectric focusing in capillary electrophoresis

✍ Scribed by Mingde Zhu; David L. Hansen; Sam Burd; Frank Gannon

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 558 KB
• Volume: 480
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84300-4

✦ Synopsis

The electrophoretic mobility of charged molecules in a free solution depends on their average charge, mass and shape, and the properties of the solvent media. In addition, differences in solvent media properties usually alter the average charge and shape of the charged molecules or form complexes with the molecules, substantially changing their electrophoretic mobility. In this study, we report the effect of the electrophoretic sample injection, the use of polymer modifiers in the running buffer, and the presence of organic modifiers on the separation of proteins, peptides and DNA molecules in free zone electrophoresis.

We also report on the effect of ampholytes and salt concentrations in free zone isoelectric focusing. To reduce convective disturbances in free zone electrophoresis at high field strength, the experiments were run in narrow-bore 25-50-pm I.D. fused-silica capillary tubes. The optical path of the UV detector was designed to increase sensitivity.