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Analysis of acrylamido-buffers for isoelectric focusing by capillary zone electrophoresis

โœ Scribed by Prof. Pier Giorgio Righetti; Claudia Ettori; Marcella Chiari

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
449 KB
Volume
12
Category
Article
ISSN
0173-0835

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Integration of capillary isoelectric foc
Integration of capillary isoelectric focusing with monolithic immobilized pH gradient, immobilized trypsin microreactor and capillary zone electrophoresis for on-line protein analysis
โœ Tingting Wang; Junfeng Ma; Guijie Zhu; Yichu Shan; Zhen Liang; Lihua Zhang; Yuku ๐Ÿ“‚ Article ๐Ÿ“… 2010 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 216 KB

## Abstract An integrated platform consisting of protein separation by CIEF with monolithic immobilized pH gradient (Mโ€IPG), onโ€line digestion by trypsinโ€based immobilized enzyme microreactor (trypsinโ€IMER), and peptide separation by CZE was established. In such a platform, a tee unit was used not