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Separation of arginase isoforms by capillary zone electrophoresis and isoelectric focusing in density gradient column

✍ Scribed by Mercedes M. Pedrosa; Prof. Maria Estrella Legaz

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 858 KB
Volume: 16
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.11501601106

No coin nor oath required. For personal study only.

✦ Synopsis

Four major arginase isoforms, I, 11, I11 and IV, have been detected in Evernia prunastri thallus. They differ in terms of both physical and biochemical properties. The isoelectric point (pZ) of these proteins has been determined by both isoelectric focusing in density gradient column and high-performance capillary electrophoresis (HPCE). Isoelectric focusing revealed charge microheterogeneity for isoforms I1 and IV whereas arginases I and I1 had the same pfvalue of 5.8. HPCE separation confirmed this charge microheterogeneity for isoform IV but not for isoform 111, and provided evidence of microheterogneity for isoforms I and 11. The effect of various electrolyte buffers and running conditions on the HPCE separation of arginase isoform were investigated. Addition of 0.5 mM spermidine (SPD) to the running buffer reduced the electroosmotic flow (EOF) and permitted discriminating between the native proteins and protein fragments.

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