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Extraction of starch by dimethyl sulfoxide and quantitation by enzymatic assay

โœ Scribed by Nicholas C. Carpita; Jan Kanabus

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
620 KB
Volume
161
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A method for the rapid, sensitive, and specific determination of starch in plant tissues is described. Starch from a variety of plant tissues is solubilized by stirring for 24 h or by sonication for 40 min in dimethyl sulfoxide. Dilution of this extract to less than 20% dimethyl sulfoxide permits a nearly complete hydrolysis of the starch in less than 3 h with glucoamylase from Rhizopus niveus. Quantitation of liberated glucose by a coupled hexokinase and glucose-6-phosphate dehydrogenase method provides an additional degree of specificity.

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## Abstract Complete dissolution is needed for the separation, characterization, or homogeneous labeling of whole starch molecules. A method is presented to quantify the extent of starch dissolution in DMSO for the first time; it is validated on a commercial rice starch. It is used directly on star