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Extracellular α-amylase fromThermus filiformisOrk A2: purification and biochemical characterization

✍ Scribed by M. C. V. Egas; M. S. da Costa; Don A. Cowan; Euclides M. V. Pires


Publisher
Springer
Year
1998
Tongue
English
Weight
365 KB
Volume
2
Category
Article
ISSN
1431-0651

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The present study was aimed to express in Escherichia coli of an extracellular lipase from a local soil isolate. The lipase gene from soil isolate bacillus subtilis strain was cloned previously. The cloned gene in pTZ57R was digested out using NdeI and BamHI restriction enzymes. The extracted insert