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Extracellular polysaccharide of Erwinia chrysanthemi

✍ Scribed by James S.S. Gray; Theodore A.W. Koerner; Rex Montgomery

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
336 KB
Volume
266
Category
Article
ISSN
0008-6215

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✦ Synopsis

The extracellular polysaccharide (EPS) produced by E. chrysanthemi pv. zeae strain SR260, a strain pathogenic to corn, has a branched hexasaccharide repeat unit consisting of a glucomannorhamnan backbone [ ---, 3)-/3-D-Glcp-( 1 ---, 4) -t~-D-Manp-( 1 ---, 3)-t~-L-Rhap-(1 ~ ] and a trisacchadde side-chain [C~-L-Rhap-(1---,3)-~-L-Rhap-(1---,4)?-GlcAp-( 1 ~ ] attached to 0-3 of the mannose in the backbone [ 1 ]. All the anomeric configurations, except that of the rhamnosyl-glucosyluronic linkage in the side chain, were unambiguously assigned by a combination of 1 and 2D NMR spectroscopy. The tentative assignment of the rhamnosyl-ghcosyluronic glycosyl linkage as Ot-L is now proven.

πŸ“œ SIMILAR VOLUMES

Isolation and characterization of a recA
Isolation and characterization of a recA mutant of Erwinia chrysanthemi B374
✍ Faelen, Michel ;Schoonejans, Eric ;Toussaint, Ariane πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 323 KB

Cosmids carrying a rec gene of the Erwinia chrysanthemi strain B 374 were identified in a genomic library constructed by Reverchon et al. (1985). One of them was mutagenized with transposon Tnl and the rec: : T n l mutation was introduced into the B 374 chromosome, providing the first rec-less E. ch

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✍ Hedegaard, Lisbeth ;Danchin, Antoine πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 560 KB

A DNA fragment containing the cya gene region ofErwinia chrysanthemi, B374 was cloned in vivo and transferred into cells of E. coli using a plasmid pULB113 derived from RP4 followed by subcloning in vitro into the vector pBR322. The cya gene encodes a 95 kDal protein that complemented E. coli cya mu