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Expression pattern of stop lacZ reporter gene in adult and developing mouse brain

✍ Scribed by Alice Couégnas; Annie Schweitzer; Annie Andrieux; M. Said Ghandour; Nelly Boehm

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
853 KB
Volume
85
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Stable tubulin‐only polypeptide (STOP) proteins are microtubule‐associated proteins responsible for microtubule stabilization in neurons. STOP null mice show apparently normal cerebral anatomy but display synaptic defects associated with neuroleptic‐sensitive behavioral disorders. STOP null mice have therefore been proposed as an animal model for the study of schizophrenia. In the present study, the expression pattern of STOP gene in developing and adult brain has been examined by using lacZ gene inserted in the STOP locus, as a reporter gene. β‐Galactosidase (β‐gal) immunostaining was confined to neuronal cells and projections. Strong labeling was observed in the whole olfactory system, cortical layer VII, hippocampus, hypothalamus, cerebellum, habenula, fasciculus retroflexus, and interpeduncular nucleus in adults. Additionally, ventral thalamic nucleus, clusters of positive cells in striatum, and Cajal‐Retzius cells of cortical layer I were labeled in young mice. The strong expression of STOP lacZ reporter gene observed in brain is confined to areas that may be involved in the schizophrenia‐related symptoms observed in STOP‐deficient mice. © 2007 Wiley‐Liss, Inc.

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