Targeted insertion of a lacZ reporter gene into the mouse Cer1 locus reveals complex and dynamic expression during embryogenesis
✍ Scribed by Edouard G. Stanley; Christine Biben; Janette Allison; Lynne Hartley; Ian P. Wicks; Ian K. Campbell; Michael McKinley; Louise Barnett; Frank Koentgen; Lorraine Robb; Richard P. Harvey
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 425 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1526-954X
No coin nor oath required. For personal study only.
✦ Synopsis
The mouse Cer1 (mCer1, Cer-l, Cerr1) gene encodes one member of a family of cytokines structurally and functionally related to the Xenopus head-inducing factor, Cerberus (xCer). We generated a mouse line in which the Cer1 gene was inactivated by replacing the first coding exon with a lacZ reporter gene. Mice homozygous for this allele (Cer1 lacZ ) showed no apparent perturbation of embryogenesis or later development. However, the lacZ reporter revealed a number of hitherto uncharacterised sites of Cer1 expression in late fetal and adult tissues. Preliminary analysis suggests that Cer1 is not essential for their morphogenesis, differentiation, or homeostasis. genesis 26:259 -264, 2000.