Expression of tumorigenic potential in isologous hybrid clones of chinese hamster cells

✍ Scribed by M. G. Blanchard; G. Barski; B. Léon; D. Hémon

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: French
• Weight: 510 KB
• Volume: 11
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910110121

✦ Synopsis

Abstract

Karyotypes and phenotypic characteristics of several clones, developed from a wild population of somatic hybrids obtained by crossing two Chinese Hamster cell lines, were analysed in detail. The crossed cell lines were: (1) the DC‐3F, sensitive to Actinomycin D (AD) and highly tumorigenic when checked by inoculation into the Syrian Hamster cheek pouch, (2) the DC‐3F/AD/Aza, resistant to AD and non tumorigenic. In the 6 clonal lines and their 3 derivatives, obtained secondarily from tumors, the resistance to AD was mostly associated with low or even entirely non‐expressed tumorigenic properties, whereas sensitivity to the toxic action of AD was, as a rule, linked with high malignancy. For all the studied clonal and derived lines a general tendency for chromosomal deletion from the “ideal”, complete integration of the two parental karyotypes, was observed. This deletion was frequently, but not always, more accentuated following passage through the animal and production of tumors. The relatively stable aspect of the Chinese Hamster karyotype, with most of the chromosomes easily identifiable, permitted a detailed analysis of the frequency of presence or deletion in the 8 pairs and groups of autosomes, the × and the M1 abnormal marker chromosomes. This analysis has shown that, in general, a preferential deletion occurred in the chromosomes Nos. 2, 4, 5, X, and M1. However, no link existed between the frequency of these individualized chromosomal deletions and either the malignancy or the resistance to AD of the hybrid clones.