Using a set of overlapping deletion mutants in the tetracycline-resistance transposon Tn10, it has been established that certain regions of the Tn10 genome exert a powerful inhibition on translocation of an intact Tn10 element into the bacterial genome. Such inhibition is strongly temperature depend
Expression of theCellulomonas fimicellulase genescexandcenAfrom the divergenttetpromoters of transposon Tn10
โ Scribed by Neena Din; C. F. Beck; R. C. Miller; D. G. Kilburn; R. A. J. Warren
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 480 KB
- Volume
- 153
- Category
- Article
- ISSN
- 0302-8933
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โฆ Synopsis
A cartridge was constructed which contained the divergent tet promoters of transposon Tn10 between an exoglucanase gene (cex) and an endoglucanase gene (cenA) of Cellulomonas fimi. When carried in a broad-host-range vector, the cartridge gave expression of cex and cenA in Escherichia coli, Rhodobacter capsulatus and Klebsiella pneumoniae.
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