✦ LIBER ✦

The tetracycline-resistance transposon Tn10 inhibits translocation of Tn10

✍ Scribed by Beck, Christoph F. ;Moyed, Hilary ;Ingraham, John L.

Publisher: Springer
Year: 1980
Tongue: English
Weight: 289 KB
Volume: 179
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425476

No coin nor oath required. For personal study only.

✦ Synopsis

Using a set of overlapping deletion mutants in the tetracycline-resistance transposon Tn10, it has been established that certain regions of the Tn10 genome exert a powerful inhibition on translocation of an intact Tn10 element into the bacterial genome. Such inhibition is strongly temperature dependent: at 37 degrees C translocation is inhibited by at least a factor of 100; no inhibition of translocation is detected at 30 degrees C.

📜 SIMILAR VOLUMES

Insertion of the tetracycline resistance

Insertion of the tetracycline resistance translocation unit Tn10 in thelac operon ofEscherichia coli K12

✍ Foster, T. J. 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 424 KB

Analysis of the reduction in expression

Analysis of the reduction in expression of tetracycline resistance determined by transposon Tn10 in the multicopy state

✍ Coleman, D. C. ;Foster, T. J. 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 815 KB

Characterization of Tn10d-Cam: A transpo

Characterization of Tn10d-Cam: A transposition-defective Tn10 specifying chloramphenicol resistance

✍ Elliott, Thomas ;Roth, John R. 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 844 KB

We have constructed a small, transposition-defective derivative of the transposon Tn10 that carries the chloramphenicol acetyltransferase gene of pACYC184. This new genetic element, Tn10d-Cam, transposes when Tn10 transposase is provided from a multi-copy plasmid. Transposon insertion mutagenesis of

Transposition of the kanamycin-resistanc

Transposition of the kanamycin-resistance transposon Tn903

✍ Young, Ry ;Grillo, Dana Smith ;Isberg, Ralph ;Way, Jeffrey ;Syvanen, Michael 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 874 KB

Expression of theCellulomonas fimicellul

Expression of theCellulomonas fimicellulase genescexandcenAfrom the divergenttetpromoters of transposon Tn10

✍ Neena Din; C. F. Beck; R. C. Miller; D. G. Kilburn; R. A. J. Warren 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 480 KB

A cartridge was constructed which contained the divergent tet promoters of transposon Tn10 between an exoglucanase gene (cex) and an endoglucanase gene (cenA) of Cellulomonas fimi. When carried in a broad-host-range vector, the cartridge gave expression of cex and cenA in Escherichia coli, Rhodobact

Evidence for a common mechanism for the

Evidence for a common mechanism for the insertion of the Tn10 transposon and for the generation of Tn10-stimulated deletions

✍ Noel, K. Dale ;Ames, Giovanna Ferro-Luzzi 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 646 KB

Mutations in and near the Salmonella typhimurium histidine transport operon were generated by insertion of the translocatable tetracycline-resistance element Tn10. Deletion mutants affecting histidine transport genes were subsequently isolated in several of the Tn10-containing strains. Tn10 insertio