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Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10

✍ Scribed by Morona, Renato ;Reeves, Peter

Publisher: Springer
Year: 1981
Tongue: English
Weight: 394 KB
Volume: 184
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00352517

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✦ Synopsis

We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb EcoRI fragment, and Tn5 insertion mutagenesis locates the gene near one end of this EcoRI fragment. An EcoRI-PstI fragment has been subcloned into pBR322 to facilitate further analysis of the gene.

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