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Expression of the Epstein-Barr virus DNA polymerase in Escherichia coli for use as antigen for the diagnosis of nasopharyngeal carcinoma

✍ Scribed by Lung-Shen Lin; Lee-Hwa Ro; Mu-Shung Lo; Wan-Ling Huang; Jeng Ma; Tong-Hsuan Chang; Chih-Hung Shu; Kuan-Chih Chow; Wu-Tse Liu; Kuang Y. Chen; Huey-Lang Yang

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
722 KB
Volume
45
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Epstein‐Barr virus (EBV) encoded DNA polymerase (POL) was cloned and over‐expressed in Escherichia coli. Western blot analysis confirmed the presence of antibody to this POL protein in sera from nasopharyngeal carcinoma (NPC) patients. By Western blot analysis, moderate to high concentration of IgG POL‐specific antibodies were present in 43 of 48 NPC sera and only 4 of 48 healthy, seropositive controls. The POL‐specific IgG antibodies appear as early as stage I of NPC, suggesting that the recombinant POL protein can be a useful diagnostic marker for early diagnosis of the disease. It was also found that human sera containing high titer of cytomegalovirus (CMV) antibodies or herpes simplex virus type 1 (HSV‐1) antibodies did not cross‐react with the recombinant EBV POL, despite the homology shared by DNA polymerase proteins of these viruses. © 1995 Wiiey‐Liss, inc.

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