For future structural and functional genomics programs new tools will be required. The implementation of high-throughput (HTP) methods for protein production will be an essential element. Present HTP protein production developments in structural genomics are aimed at obtaining well-expressing and hi
β¦ LIBER β¦
Expression of soluble recombinant proteins in a cell-free system using a 96-well format
β Scribed by Didier Busso; Rosalind Kim; Sung-Hou Kim
- Book ID
- 117331317
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 201 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0165-022X
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## Abstract In this work, it was discovered that the stability of mRNA in a cellβfree extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequ
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