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A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

✍ Scribed by Hanna-Riikka Kärkkäinen; Hanna P Lesch; Antti I Määttä; Pyry I Toivanen; Anssi J Mähönen; Miia M Roschier; Kari J Airenne; Olli H Laitinen; Seppo Ylä-Herttuala

Book ID
115024762
Publisher
BioMed Central
Year
2009
Tongue
English
Weight
862 KB
Volume
2
Category
Article
ISSN
1756-0500

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## Abstract The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to adapt to a multi‐parallel process. We have developed a bacmid vector that does not require any for