Expression of oncogenes and tumor suppressor genes in human hepatocellular carcinoma and hepatoblastoma cell lines

✍ Scribed by Mahmood Farshid; Dr. Edward Tabor

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 592 KB
• Volume: 38
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890380402

✦ Synopsis

Abstract

The expression of nine oncogenes (c‐myc, N‐myc, N‐ras, H‐ras, k‐ras, abl, fos, src, and raf) and two tumor suppressor genes (p53 and RB) were studied by northern blot hybridization in six human hepatocellular carcinoma or hepatoblastoma cell lines (PLC/PRF/5, HepSB, Hep G2, 2.2.15, HLE, and HLF) and in a human embryonic lung fibroblast cell line (WI‐38) to look for differences that might be associated with the presence (PLC/PRF/5, Hep3B, and 2.2.15) or absence (Hep G2, HLE, and HLF) of integrated hepatitis B virus (HBV) DNA. The levels of expression of the oncogenes and tumor suppressor genes were unrelated to the presence or absence of integrated HBV‐DNA. Furthermore, the intensity of expression of these oncogenes was no greater in the 2.2.15 cell line (consisting of Hep G2 cells transfected with hepatitis B virus) than in untransfected Hep G2 cells. © 1992 Wiley‐Liss, Inc.