Mammalian cell expression cloning has become a standard technique for the isolation of mammalian genes or cDNAs. Its advantage is that the biological functions of the gene of interest are used for cloning. Therefore, the identified cDNAs or genes should be functional in vivo, and there is no need fo
Expression of mammalian cell-surface receptors in higher eukaryotic systems
โ Scribed by Michael J. Browne; Joanna M. Balcarek
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 576 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0958-1669
No coin nor oath required. For personal study only.
โฆ Synopsis
The study of recombinant receptors has progressed rapidly over the past year. These receptors are complex, often multimeric, protein systems. We have seen further progress in the use of mammalian cell lines, but two areas of development in receptor expression are notable: first, increasing use and success of the baculovirus system; and second, improved, rapid methods for detecting signal transduction.
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