✦ LIBER ✦

Use of mammalian cell expression cloning systems to identify genes for cytokines, receptors, and regulatory proteins

✍ Scribed by Naoki Nakayama; Takashi Yokota; Ken-ichi Arai

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 855 KB
Volume: 3
Category: Article
ISSN: 0958-1669
DOI: 10.1016/0958-1669(92)90077-v

No coin nor oath required. For personal study only.

✦ Synopsis

Mammalian cell expression cloning has become a standard technique for the isolation of mammalian genes or cDNAs. Its advantage is that the biological functions of the gene of interest are used for cloning. Therefore, the identified cDNAs or genes should be functional in vivo, and there is no need for physical or chemical information about the gene products, so that protein purification in sufficient quantity to raise antibodies or to obtain amino acid sequences is not necessary. Here, we summarize recent progress in mammalian cell cloning systems, and discuss the possible directions in which this technique will lead.

📜 SIMILAR VOLUMES

A Chimeric Reporter Gene Allowing for Cl

A Chimeric Reporter Gene Allowing for Clone Selection and High-Throughput Screening of Reporter Cell Lines Expressing G-Protein-Coupled Receptors

✍ Knut Kotarsky; Christer Owman; Björn Olde 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 114 KB

Efficient screening of ligands interacting with Gprotein-coupled receptors is central for modern drug development. Here, we describe an optimized reporter vector primarily intended for use in reporter cell lines expressing such receptors. The construct consists of a synthetic enhancer containing 9؋