Expression of MAGE genes in osteosarcoma

✍ Scribed by Takao Sudo; Terukazu Kuramoto; Setsuro Komiya; Akio Inoue; Kyogo Itoh

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 494 KB
• Volume: 15
• Category: Article
• ISSN: 0736-0266
• DOI: 10.1002/jor.1100150119

✦ Synopsis

Abstract

Expression of MAGE genes that encode tumor‐rejection antigens recognized by cytotoxic T lymphocytes with major histocompatibility complex class‐I antigens was investigated in human osteosarcomas (20 cell lines and eight fresh tumor tissues). MAGE‐1,2,3,4, and 6 genes were expressed at the mRNA level in 11(52.4%), 10(47.6%), 10(47.6%), one (4.8%) and 10 (47.6%) of 21 tumor cell lines, respectively, and in five (62.5%), six (75%), five (62.5%), one (12.5%) and five (62.5%) of eight fresh tumor tissues as determined by the reverse transcription‐polymerase chain reaction method. MAGE‐1 or 4 protein was detected by immunoblot analysis in eight of 11 or one of one tumor cell lines, respectively where it was expressed at the mRNA level. Major histocompatibility complex class‐I antigens were expressed 19 of 21 tumor cell lines. These results suggest that MAGE tumor‐rejection antigens are expressed in substantial numbers of osteosarcomas in a major histocompatibility class‐I‐restricted manner.