Expression of human lymphotoxin in Namalwa KJM-1 cells adapted to serum-free medium

✍ Scribed by Hiromasa Miyaji; Nahoko Harada; Tamio Mizukami; Seiji Sato; Nobuo Fujiyoshi; Seiga Itoh

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 293 KB
• Volume: 4
• Category: Article
• ISSN: 0920-9069
• DOI: 10.1007/bf00148809

✦ Synopsis

A Namalwa cell line, KJM-1, which was adapted to serum-free medium is thought to be a good host cell line for recombinant DNA technology. We previously reported the expression of human g-interferon (B-IFN) in Namalwa KJM-1 (Miyaji, 1989a). The utility of Namalwa KJM-1 for expression of foreign genes was further examined. As a target gene to be expressed, human lymphotoxin (hLT) cDNA was used. It was engineered for expression in Namalwa KJM-1 using a simian virus 40 (SV40)-based expression vector pAGE107 (Miyaji, 1989a). It contains all components necessary for the expression of cDNA in mammalian cells. The expression vector was introduced into Namalwa KJM-1 by electroporation. Among the transformants, clone 7 was further examined for the expression of hLT in serum-free medium. The production level of hLT was augmented with the increase of the cell density. Thus it was further indicated that Namalwa KJM-1 is useful for production of foreign gene products.