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High-level expression of recombinant human soluble thrombomodulin in serum-free medium by CHO-K1 cells

โœ Scribed by Masaaki Ogata; Ken-ichi Wakita; Kazuo Kimura; Yasumasa Marumoto; Kiyomoto Oh-i; Shin Shimizu

Publisher
Springer
Year
1993
Tongue
English
Weight
863 KB
Volume
38
Category
Article
ISSN
1432-0614

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Efficient expression of human beta-inter
Efficient expression of human beta-interferon in Namalwa KJM-1 cells adapted to serum-free medium by a dhfr gene coamplification method
โœ Hiromasa Miyaji; Nahoko Harada; Tamio Mizukami; Seiji Sato; Nobuo Fujiyoshi; Sei ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 563 KB

We previously reported the expression of human beta-interferon (B-IFN) (Miyaji et al., 1989) and human lymphotoxin (Miyaji et al., 1990) in Namalwa KJM-1 cells adapted to serum-free medium. To establish an efficient gene expression system, a dihydrofolate reductase (dhfr) gene coamplification method