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Expression of green fluorescent protein in Lactococcus lactis

✍ Scribed by Pilar Fernández de Palencia; Concha Nieto; Paloma Acebo; Manuel Espinosa; Paloma López

Book ID
117519686
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
434 KB
Volume
183
Category
Article
ISSN
0378-1097

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## Abstract __Lactococcus lactis__ is a promising host for (membrane) protein overproduction. Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in __L. lactis__. Incorporation efficiencies of SeMet in the membrane protein complex OpuA (an ABC transpo