Eukaryotic membrane protein overproduction in Lactococcus lactis
✍ Scribed by Edmund RS Kunji; Ka Wai Chan; Dirk Jan Slotboom; Suzanne Floyd; Rosemary O’Connor; Magnus Monné
- Book ID
- 108105287
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 268 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0958-1669
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## Abstract __Lactococcus lactis__ is a promising host for (membrane) protein overproduction. Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in __L. lactis__. Incorporation efficiencies of SeMet in the membrane protein complex OpuA (an ABC transpo
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