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Expression of bovine trypsin in Lactococcus lactis

✍ Scribed by Liyan Yao; Chaoxin Man; Feng Zhao; Xingguang Qu; Xiyan Han; Daqing Sun; Yujun Jiang

Book ID
116540549
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
207 KB
Volume
20
Category
Article
ISSN
0958-6946

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## Abstract __Lactococcus lactis__ is a promising host for (membrane) protein overproduction. Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in __L. lactis__. Incorporation efficiencies of SeMet in the membrane protein complex OpuA (an ABC transpo