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Expression of functional Thermoplasma acidophilum proteasomes in Escherichia coli

✍ Scribed by Peter Zwickl; Friedrich Lottspeich; Wolfgang Baumeister

Book ID
115926602
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
403 KB
Volume
312
Category
Article
ISSN
0014-5793

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Gene libraries from the magnetotactic bacterium, Aquaspirillum magnetotacticum were constructed in Escherichia coli with cosmids pLAFR3 and c2RB as vectors. Recombinant cosmids able to complement the thr-1, leuB, and proA mutations of the host were identified. The Pro+ recombinant cosmid restored wi