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Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12

✍ Scribed by Waleh, Nahid S.

Publisher
Springer
Year
1988
Tongue
English
Weight
544 KB
Volume
214
Category
Article
ISSN
0026-8925

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✦ Synopsis

Gene libraries from the magnetotactic bacterium, Aquaspirillum magnetotacticum were constructed in Escherichia coli with cosmids pLAFR3 and c2RB as vectors. Recombinant cosmids able to complement the thr-1, leuB, and proA mutations of the host were identified. The Pro+ recombinant cosmid restored wild-type phenotype in proA and proB but not in the proC mutants of E. coli. The results of restriction endonuclease digestion and Southern hybridization analysis indicate that the relevent leu and pro biosynthetic genes of A. magnetotacticum are not closely linked on the chromosome.

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