✦ LIBER ✦

Functional expression of the genes of Escherichia coli in gram-positive Corynebacterium glutamicum

✍ Scribed by Ozaki, Akio ;Katsumata, Ryoichi ;Oka, Tetsuo ;Furuya, Akira

Publisher: Springer
Year: 1984
Tongue: English
Weight: 397 KB
Volume: 196
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00334113

No coin nor oath required. For personal study only.

✦ Synopsis

Hybrid plasmids were constructed by combining in vitro the Escherichia coli plasmid pGA22, which carries the genes determining resistance to kanamycin, tetracycline, chloramphenicol and ampicillin, with the cryptic plasmids, pCG1 and pCG2, of Corynebacterium glutamicum. The hybrid plasmids were introduced into C. glutamicum and E. coli and replicated in both hosts. They expressed all the E. coli resistance phenotypes except ampicillin resistance in C. glutamicum. The levels of antibiotic inactivating enzymes encoded on these plasmids were about four to ten times lower in C. glutamicum than in E. coli. Despite the lack of expression of ampicillin resistance, beta-lactamase activity was detected in C. glutamicum carrying hybrid plasmids.

📜 SIMILAR VOLUMES

Functional expression of Aquaspirillum m

Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12

✍ Waleh, Nahid S. 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 544 KB

Gene libraries from the magnetotactic bacterium, Aquaspirillum magnetotacticum were constructed in Escherichia coli with cosmids pLAFR3 and c2RB as vectors. Recombinant cosmids able to complement the thr-1, leuB, and proA mutations of the host were identified. The Pro+ recombinant cosmid restored wi

Expression of the recA gene of Escherich

Expression of the recA gene of Escherichia coli in several species of gram-negative bacteria

✍ de Henestrosa Fernandez, Antoni R. ;Calero, Sebastian ;Barbé, Jordi 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 399 KB

A broad host range plasmid containing an operon fusion between the recA and lacZ genes of Escherichia coli was introduced into various aerobic and facultative gram-negative bacteria-30 species belonging to 20 different genera - to study the expression of the recA gene after DNA damage. These include

Expression of the transglutaminase gene

Expression of the transglutaminase gene in Escherichia coli

✍ Dominic W.S. Wong; Carl A. Batt; John E. Kinsella 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 935 KB

Expression of ribosomal protein genes in

Expression of ribosomal protein genes in Escherichia coli

✍ Cabezón, Teresa ;Faelen, Michel ;De Wilde, Michel ;Bollen, Alex ;Thomas, René 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 330 KB

Streptomycin or spectinomycin treatment of an E. coli strain, carrying the strR and spcR alleles on the chromosome and the wild-type (sensitive) alleles on the episome, selects for inactivation of the relevant sensitive allele. After Mu induced mutagenesis, in the absence of selection against extend

The phosphoenolpyruvate carboxylase gene

The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequence, and expression

✍ Eikmanns, Bernhard J. ;Follettie, Max T. ;Griot, Martin U. ;Sinskey, Anthony J. 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 935 KB

Hek: an Escherichia coli function invove

Hek: an Escherichia coli function invoved in functional expression of the kil gene of bacteriophage Mu

✍ Goosen, N. ;van de Putte, P. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 247 KB

An Escherichia coli mutant has been isolated (Hek) in which the kil gene of bacteriophage Mu is not functionally expressed. The hek locus has been mapped between rpoD (66.2 min) and argR (69.5 min) on the E. coli chromosome. No influence of the hek mutation on phage or E. coli development could be d