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Expression of functional Bacillus SpoIISAB toxin–antitoxin modules in Escherichia coli

✍ Scribed by Patrik Florek; Katarína Muchová; Pamela Pavelčíková; Imrich Barák

Book ID
109331003
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
352 KB
Volume
278
Category
Article
ISSN
0378-1097

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We have cloned a 14 kb DNA segment containing the coding sequence (polC) for DNA polymerase III (PolIII) from the Bacillus subtilis chromosome. The plasmid carrying the sequence, pRO10, directs the synthesis of the 160 kDa PolIII protein and three additional polypeptides in Escherichia coli maxicell