✦ LIBER ✦

Expression of cell surface determinant(s) on murine myeloma stem cells and hematopoietic stem cells

✍ Scribed by Michael J. Daley; Thomas Williams; Noel L. Warner

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 799 KB
Volume: 14
Category: Article
ISSN: 0014-2980
DOI: 10.1002/eji.1830141206

No coin nor oath required. For personal study only.

✦ Synopsis

Murine B cell lymphomas and myelomas were examined for the expression of a determinant previously found exclusively on normal pluripotent stem cells colonyforming unit-spleen (CFU-s). This determinant(s), which is defined by a rabbit antimouse brain antiserum (RaMB), is present on the tumor stem cell population of some but not all B cell neoplasms examined. The determinant is not detected on tumor cells of the macrophage or T cell lineage. Absorption of the activity in RaMB with myeloma cells, concomitantly removed reactivity with the normal stem cell, CFU-s, and the myeloma stem cell, plasmacytoma CFU-s. Sorting analysis further showed that the antigen was diminished within a positive tumor population as cells acquired the capacity to secrete immunoglobulin. These studies suggest that this normal stem cell-associated antigen may also be an early differentiation antigen for the B cell lineage, and is expressed on some stem cells of B cell tumors.

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