Demonstration of a hematopoietic stem cell antigen (SC-1) on a murine lymphoma and isolation of variants lacking the antigen

✍ Scribed by Ross S. Basch; Thomas Panagiotatos; Joan W. Berman; Joel N. Buxbaum

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 491 KB
• Volume: 107
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041070309

✦ Synopsis

Abstract

Murine multipotential hematopoietic stem cells (CFU‐s) bear an antigen (SC‐1) which is recognized by heterologous antisera to mouse brain. We have found that cloned Thy‐1 negative variants of the T‐cell lymphoma RL ♂1 are sensitive to complement‐mediated cytolysis by anti‐brain serum and can absorb the anti‐stem cell activity from the antiserum. We have isolated several subclones derived from a primary Thy‐1 negative variant which are not susceptible to anti‐brain serum. The surface of the resistant lines has little or no antigen capable of binding anti‐mouse brain antibodies as measured by either immunofluorescence or a radioimmunoassay. These lines are also unable to absorb the anti‐bodies responsible for the cytotoxic effect of rabbit anti‐mouse brain serum against CFU‐s. We conclude that the predominant antigen, serologically detectable on Thy‐1 negative variants of RL ♂1, is SC‐1.