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Regulated expression of transgenes in embryonic stem cell-derived neural cells

✍ Scribed by David S. Lorberbaum; David Gottlieb

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
414 KB
Volume
49
Category
Article
ISSN
1526-954X

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✦ Synopsis

Abstract

Discovery and characterization of gene promoters, enhancers and repressor binding elements is an important research area in neuroscience. Here, the suitability of embryonic stem cells and their neural derivatives as a model system for this research is investigated. Three neural transgenic constructs (from the Mnx1, Fabp7, and tuba1a genes) that have been validated in transgenic mice were inserted into embryonic stem cells as stable transgenes. These transgenic embryonic stem cells were differentiated into neural cultures and the pattern of transgene expression across a series of inducing conditions determined. The pattern of expression matched that predicted from transgenic mouse experiments for each of the three transgenes. The results show that embryonic stem cells and their neural derivatives comprise a promising model for investigating the mechanisms that control cell‐ and temporal‐specific neural gene transcription. genesis 49:66–74, 2011. Β© 2010 Wiley‐Liss, Inc.

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Corrigendum 1, regarding Figure 1 of the published article: in panel B, the position of the label ''Ctcf'' is not properly aligned. The figure should appear as follows: Corrigendum 2, regarding Figure 2 of the published article: in the figure key, the denotation of the line-symbol indicating repress